Información del producto
Shc (phospho Tyr427) rabbit pAb
Información adicional
Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human Shc around the phosphorylation site of Tyr427. AA range:393-442
Subcellular Location
Cytoplasm.
[Isoform p46Shc]: Mitochondrion matrix . Localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p6
[Isoform p66Shc]: Mitochondrion . In case of oxidative conditions, phosphorylation at 'Ser-36' of isoform p66Shc, leads to mitochondrial accumulation. .
[Isoform p46Shc]: Mitochondrion matrix . Localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p6
[Isoform p66Shc]: Mitochondrion . In case of oxidative conditions, phosphorylation at 'Ser-36' of isoform p66Shc, leads to mitochondrial accumulation. .
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Rat
Mouse
Rat
Human Gen
6464
Observed Band
66(p66 isoform), 52(p52 isoform), 46(p46 isoform)kD
Other Name
SH2 domain protein C1
SHC
SHC-transforming protein 1
SHC-transforming protein 3
SHC-transforming protein A
SHC1
SHCA
Src homology 2 domain-containing-transforming protein C1
SHC
SHC-transforming protein 1
SHC-transforming protein 3
SHC-transforming protein A
SHC1
SHCA
Src homology 2 domain-containing-transforming protein C1
Background
This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen