Información del producto
Akt2 (phospho Ser474) rabbit pAb
Información adicional
Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human Akt2 around the phosphorylation site of Ser474. AA range:432-481
Subcellular Location
Cytoplasm. Nucleus. Cell membrane
Peripheral membrane protein. Early endosome . Localizes within both nucleus and cytoplasm of proliferative primary myoblasts and mostly within the nucleus of differentiated primary myoblasts. By virtue of the N-terminal PH domain, is recruited to sites of
Peripheral membrane protein. Early endosome . Localizes within both nucleus and cytoplasm of proliferative primary myoblasts and mostly within the nucleus of differentiated primary myoblasts. By virtue of the N-terminal PH domain, is recruited to sites of
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Rat
Mouse
Rat
Human Gen
208
Observed Band
56kD
Other Name
AKT2
PKB beta
Protein kinase Akt-2
Protein kinase B beta
RAC-PK-beta
RAC-beta serine/threonine-protein kinase
PKB beta
Protein kinase Akt-2
Protein kinase B beta
RAC-PK-beta
RAC-beta serine/threonine-protein kinase
Background
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 o