Información del producto
Akt1 (phospho Ser246) rabbit pAb
Información adicional
Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Ser246. AA range:212-261
Subcellular Location
Cytoplasm . Nucleus . Cell membrane . Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane whe
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Rat
Mouse
Rat
Human Gen
207
Observed Band
56kD
Other Name
AKT1
PKB
PKB
PKB alpha
Protein kinase B
Protein kinase B alpha
Proto-oncogene c-Akt
RAC
RAC-PK-alpha
RAC-alpha serine/threonine-protein kinase
PKB
PKB
PKB alpha
Protein kinase B
Protein kinase B alpha
Proto-oncogene c-Akt
RAC
RAC-PK-alpha
RAC-alpha serine/threonine-protein kinase
Background
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific,