Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Applications
WB, IHC
Concentration
1mg/mL
Recomended Dilution
WB 1:1,000-2,000 IHC 1:100-200
Type Clonality
Monoclonal
Immunogen
Synthetic Peptide
Subcellular Location
Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule
Iso type
IgG1
Tissue Source
Mouse
Reactivity
Human, Rat, Mouse
Human Gen
84557
Observed Band
14,16kDa
Other Name
ATG8E, MAP1LC3A,autophage-related protein LC3A
Background
Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form.