Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Applications
WB, IP, IF
Concentration
1mg/mL
Recomended Dilution
WB 1:1,000-5,000 IP 1:200 IF 1:100-200
Type Clonality
Monoclonal
Immunogen
Synthetic Peptide
Subcellular Location
Cytosol,Mitochondrion,Nucleus
Iso type
IgG1
Tissue Source
Mouse
Reactivity
Human, Rat, Mouse
Human Gen
842
Observed Band
47(51)kDa
Other Name
APAF 3 antibody, Apoptosis related cysteine peptidase antibody, Apoptotic protease-activating factor 3 antibody, CASP 9 antibody
Background
Caspase-9 is an initiator caspase, encoded by the CASP9 gene.Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).