Size
100µg
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Applications
ELISA, IF, WB
Target
HIF1A
Research Area
Signal Transduction, Metabolism, Cardiovascular, Developmental biology, Neuroscience, Stem Cells
Form
liquid
Recomended Dilution
WB: 1:1000-1:10000
Type Clonality
monoclonal
Immunogen
hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)
Minimum Purity
≥95% as determined by SDS-PAGE
Synonyms
Hypoxia-inducible factor 1-alpha (HIF-1-alpha, HIF1-alpha)|ARNT-interacting protein|Basic-helix-loop-helix-PAS protein MOP1|Class E basic helix-loop-helix protein 78 (bHLHe78)|Member of PAS protein 1|PAS domain-containing protein 8|HIF1A|BHLHE78|MOP1|PASD
Host
Mouse
UniProt ID
Q16665
Gene ID
3091
Purification
protein A+G purified
Clone Number
5H12
Iso type
IgG1
Reactivity
Human
Observed MW
120 kDa
Background
Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydr