Multiplex Assay Kit for Activated Protein C (APC) ,etc. by FLIA (Flow Luminescence Immunoassay)
Ref: CC-LMA738Mu
96T
Información del producto
Multiplex Assay Kit for Activated Protein C (APC) ,etc. by FLIA (Flow Luminescence Immunoassay)
Información adicional
Stabillity at RT
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the
Applications
FLIA Kit for Antigen Detection.
Detection Range
4.88-5000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 1.627 pg/mL
Specificity
No significant cross-reactivity or interference between Activated Protein C (APC) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Activated Protein C (APC) ,etc. by FLIA (Flow Luminescence Immunoassay).
Research Area
FLIA Kit for Antigen Detection.
Organism species
Mus musculus (Mouse)
Item Name
Activated Protein C
Assay Length
3h
Method
Double-antibody Sandwich
Sample Type
Plasma
Format
48T, 96T, 96T×5, 96T×10, 96T×100
Assay Procedure
Double-antibody Sandwich
Test Principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinyla
proportional to the concentration of analytes of interest in the sample.
proportional to the concentration of analytes of interest in the sample.
Precision
CV(%) = SD/meanX100
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activated Protein C (APC) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
Intra-Assay: CV<10%
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activated Protein C (APC) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment
2. Add 100μL standard or sample to each well,
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°
4. Wash plate on magnetic frame for three times
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°
6. Wash plate on magnetic frame for three times
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
C on shaker
C on shaker
C on shaker
add 10μL magnetic beads, and incubate 90min at 37°
1. Preparation of standards, reagents and samples before the experiment
2. Add 100μL standard or sample to each well,
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°
4. Wash plate on magnetic frame for three times
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°
6. Wash plate on magnetic frame for three times
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
C on shaker
C on shaker
C on shaker
add 10μL magnetic beads, and incubate 90min at 37°
UniProt ID
-