Información del producto
The Neuropeptide Y Y1 Receptor was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex, arcuate and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500 - 1/1000 in PBS - Bn/Av-HRP detection. The antibody was characterized by immunohistochemistry and Western blot. Western blot showed one immunoreactive band of 40 kD and a single high molecular weight band, presumably a precursor molecule. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human NPY Y1 receptors.
Información adicional
Size
100 µl
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot
Target
Rat NPY Y1 receptor (356-382)
Type Clonality
polyclonal
Immunogen
Rat NPY Y1 receptor (356-382)
Format
Affinity Purified
Host
Rabbit
Reactivity
Hamster, Human, Mouse, Rat
Conjugation
Unconjugated