Información del producto
The Substance P antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat substantia nigra and spinal cord using biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500-1/1000 in PBS/0.3% Triton X-100 - Cy3 Fluorchrome and 1/6000-1/8000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. The specificity of the antiserum for Substance P was demonstrated using soluble pre-adsorption with the peptides in question at a final concentration of 10 µg of peptide per mL of diluted antiserum. Substance P immunolabeling was completely abolished by pre-adsorption with Substance P. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: neurokinin A, neurokinin B, somatostatin and neuropeptide K.
Información adicional
Size
100 µl
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot
Target
Substance P
Type Clonality
polyclonal
Immunogen
Substance P
Format
Whole Serum
Host
Rabbit
Reactivity
Bird, Bullfrog, Cat, Chick, Chicken, Dog, Dolphin, Doves, Equine, Fish, Frog, Gerbil, Goat, Goldfish, Guinea Pig, Hamster, Horse, Human, Monkey, Mouse, Mudpuppy, Narwhal, Parrot, Pig, Pig, Pigeon, Quail , Rabbit, Ram (Sheep), Rat, Ratfish, Salmon, Scorpae
Conjugation
Unconjugated