The antibody has a proven strong indirect immunofluorescence at 1/400 - 1/800 and 4+ biotin-streptavidin/HRP staining at a 1/2000 - 1/4000 dilution in rat brainstem, cerebellum and adrenal medulla. Using Western blot of purified DBH the antiserum detects a triplet at approximately 72-74 kD.