ELISA Kit for Heparin (Hep) View larger

ELISA Kit for Heparin (Hep)

CC-CEV639Ge

New product

48T, 96T, 96T×5, 96T×10, 96T×100

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Data sheet

Size 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100
Storage Conditions -20°C
Shipping Conditions Entre 4°C y 8°C
Applications Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range 12.35-1,000ng/mL
Sensitivity The minimum detectable dose of this kit is typically less than 4.33ng/mL
Organism species Pan-species (General)
Alternative Names Unfractionated heparin
Item Name Heparin
Assay Length 2h
Method Competitive Inhibition
Sample Type serum, plasma and other biological fluids
Formato 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100
Test Principle This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Heparin (Hep) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Heparin (Hep) and unlab
Assay procedure summary 1. Prepare all reagents, samples and standards
UniProt ID -

More info

Especificidad:

This assay has high sensitivity and excellent specificity for detection of Heparin (Hep).
No significant cross-reactivity or interference between Heparin (Hep) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparin (Hep) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparin (Hep) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Estabilidad:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

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