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ELISA Kit for Vitamin B7 (VB7)
CC-CEG162Ge
New product
Ao comprar este produto pode ganhar até 9 pontos de fidelização. Seu carrinho totalizará 9 pontos de fidelização que podem ser convertidos num vale de desconto de 36.00EUR.
Data sheet
| Size | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Storage Conditions | -20°C |
| Shipping Conditions | Entre 4°C y 8°C |
| Applications | Enzyme-linked immunosorbent assay for Antigen Detection. |
| Detection Range | 32-20,000pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 13pg/mL |
| Organism species | Pan-species (General) |
| Alternative Names | Biotin |
| Item Name | Vitamin B7 |
| Assay Length | 2h |
| Method | Competitive Inhibition |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Formato | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique.BSA conjugated VB7 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between BSA conjugated VB7 and unlabeled VB7 (Standards or samples) with HRP |
| Assay procedure summary | 1. Prepare all reagents, samples and standards |
| UniProt ID | - |
More info
This assay has high sensitivity and excellent specificity for detection of Vitamin B7 (VB7).
No significant cross-reactivity or interference between Vitamin B7 (VB7) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin B7 (VB7) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin B7 (VB7) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.