ELISA Kit for Adrenocorticotropic Hormone (ACTH)
Ref: CC-CEA836Bo
48T, 96T, 96T×5, 96T×10, 96T×100
Información del producto
Especificidad:
This assay has high sensitivity and excellent specificity for detection of Adrenocorticotropic Hormone (ACTH).
No significant cross-reactivity or interference between Adrenocorticotropic Hormone (ACTH) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Información adicional
Size
48T, 96T, 96T×5, 96T×10, 96T×100
Storage Conditions
-20°C
Shipping Conditions
Entre 4°C y 8°C
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range
12.35-1000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 4.57pg/mL
Research Area
Endocrinology
Organism species
Bos taurus
Alternative Names
Corticotropin
Item Name
Adrenocorticotropic Hormone
Assay Length
2h
Method
Competitive Inhibition
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Format
48T, 96T, 96T×5, 96T×10, 96T×100
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adrenocorticotropic Hormone (ACTH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ad
Assay procedure summary
1. Prepare all reagents, samples and standards
UniProt ID
P01190