ELISA Kit for Corticotropin Releasing Factor (CRF)
Ref: CC-CEA835Ra
48T, 96T, 96T×5, 96T×10, 96T×100
Información del producto
Especificidad:
This assay has high sensitivity and excellent specificity for detection of Corticotropin Releasing Factor (CRF).
No significant cross-reactivity or interference between Corticotropin Releasing Factor (CRF) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Corticotropin Releasing Factor (CRF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Corticotropin Releasing Factor (CRF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Información adicional
Size
48T, 96T, 96T×5, 96T×10, 96T×100
Storage Conditions
-20°C
Shipping Conditions
Entre 4°C y 8°C
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range
12.35-1,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 5.53pg/mL
Research Area
Endocrinology
Organism species
Rattus norvegicus (Rat)
Alternative Names
CRH
Item Name
Corticotropin Releasing Factor
Assay Length
2h
Method
Competitive Inhibition
Sample Type
Serum, plasma and other biological fluids
Format
48T, 96T, 96T×5, 96T×10, 96T×100
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Corticotropin Releasing Factor (CRF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled
Assay procedure summary
1. Prepare all reagents, samples and standards
UniProt ID
P01143