HotTaq DNA polymerase catalyzes the 5'-3' synthesis of DNA. The enzyme has been proven not to have the 3' - 5'exonuclease activity. Before the first PCR step, the HotTaq DNA polymerase should be activated by 15 minute incubation at 95-97°C.
Información adicional
Size2500 Units
Storage ConditionsStore at -20C. Guaranteed stable for 6 months when properly stored.
Application KeyPCR
Supplied Product10x reaction buffer
Quality control testingFree of detectable, non-specific nucleases.
Storage Buffer20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol.