Información del producto
Alkaline Phosphatase is derived from a recombinant E.coli strain that carries the TAB5 gene. The enzyme catalyzes the dephosphorylation of 5' and 3' ends of DNA and RNA phosphomonoesters. Also, it hydrolyses ribose, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). TAB5 Alkaline Phosphatase acts on 5´ protruding, 5' recessed and blunt ends. The Phosphatase can be used in many molecular biology applications, such as cloning or probe end labeling to remove the phosphorylated ends of DNA or RNA. In cloning experiments, dephosphorylation prevents the linearized plasmid DNA from self-ligation. It can also degrade unincorporated dNTPs in PCR reactions to prepare a template for DNA sequencing. The enzyme is completely and irreversibly inactivated by heating at 70°C for 5 minutes, thereby making removal of the phosphatase prior to ligation or end labeling unnecessary.
Información adicional
Size
100 uL
Storage Conditions
Store at -20C or lower. Aliquot to avoid repeated freezing and thawing.
Application Key
IVT
Form
Liquid
Recomended Dilution
In Vitro Transcription
The optimal working dilution should be determined by the end user.
The optimal working dilution should be determined by the end user.
Storage Buffer
10 mM Tris-HCl (pH 7.4), 1 mM MgCl2 , 0.01 mM ZnCl2, 50% glycerol.