Información del producto
Mini Samples ELISA Kit for Angiotensin 1-7 (Ang1-7)
Información adicional
Stabillity at RT
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range
9.88-800pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 3.37pg/mL
Specificity
No significant cross-reactivity or interference between Mini Samples Angiotensin 1-7 (Ang1-7) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Mini Samples Angiotensin 1-7 (Ang1-7).
Organism species
Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)
Item Name
Angiotensin 1-7
Assay Length
3h
Method
Competitive Inhibition
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Due to the 100% homology of the sequence among different species, the kit can be used to detect human, mouse and rat samples.
Assay Procedure
1. Prepare all reagents, samples and standards
2. Add 25µ
3. Aspirate and wash 3 times
4. Add 50µ
5. Aspirate and wash 5 times
6. Add 50µ
7. Add 25µ
And then add 25μL prepared Detection Reagent A immediately.
C
C
C
L Stop Solution. Read at 450 nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well.
Shake and mix. Incubate 1 hour at 37°
1. Prepare all reagents, samples and standards
2. Add 25µ
3. Aspirate and wash 3 times
4. Add 50µ
5. Aspirate and wash 5 times
6. Add 50µ
7. Add 25µ
And then add 25μL prepared Detection Reagent A immediately.
C
C
C
L Stop Solution. Read at 450 nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well.
Shake and mix. Incubate 1 hour at 37°
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Angiotensin 1-7 (Ang1-7) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled
Precision
CV(%) = SD/meanX100
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Angiotensin 1-7 (Ang1-7) were tested on 3 different plates, 8 replicates in each plate.
Intra-Assay: CV<10%
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Angiotensin 1-7 (Ang1-7) were tested 20 times on one plate, respectively.
UniProt ID
-