Información del producto
High Sensitive ELISA Kit for Insulin (INS)
Información adicional
Size
48T, 96T, 96T×5, 96T×10, 96T×100
Stabillity at RT
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range
61.7-5,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 22.5pg/mL
Specificity
No significant cross-reactivity or interference between High Sensitive Insulin (INS) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of High Sensitive Insulin (INS).
Research Area
Endocrinology
Hormone metabolism
Metabolic pathway
Hormone metabolism
Metabolic pathway
Organism species
Rattus norvegicus (Rat)
Item Name
Insulin
Assay Length
3h
Method
Competitive Inhibition
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Procedure
1. Prepare all reagents, samples and standards
2. Add 50µ
3. Aspirate and wash 3 times
4. Add 100µ
5. Aspirate and wash 5 times
6. Add 90µ
7. Add 50µ
And then add 50µ
C
C
C
L Stop Solution. Read at 450 nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent A immediately.
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well.
Shake and mix. Incubate 1 hour at 37°
1. Prepare all reagents, samples and standards
2. Add 50µ
3. Aspirate and wash 3 times
4. Add 100µ
5. Aspirate and wash 5 times
6. Add 90µ
7. Add 50µ
And then add 50µ
C
C
C
L Stop Solution. Read at 450 nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent A immediately.
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well.
Shake and mix. Incubate 1 hour at 37°
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Insulin (INS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sen
Precision
CV(%) = SD/meanX100
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Insulin (INS) were tested on 3 different plates, 8 replicates in each plate.
Intra-Assay: CV<10%
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Insulin (INS) were tested 20 times on one plate, respectively.
Assay procedure summary
1. Prepare all reagents, samples and standards
UniProt ID
P01322