Información del producto
Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)
Información adicional
Size
48T, 96T, 96T×5, 96T×10, 96T×100
Stabillity at RT
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the
Storage Conditions
-20°C
Shipping Conditions
Entre 4°C y 8°C
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range
46.88-3,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 17.27pg/mL
Specificity
No significant cross-reactivity or interference between Instant Procollagen I N-Terminal Propeptide (PINP) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Instant Procollagen I N-Terminal Propeptide (PINP).
Research Area
Bone metabolism
Hepatology
Metabolic pathway
Hepatology
Metabolic pathway
Organism species
Mus musculus (Mouse)
Alternative Names
P1NP
Item Name
Procollagen I N-Terminal Propeptide
Assay Length
1h, 10min
Method
Double-antibody Sandwich
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Format
48T, 96T, 96T×5, 96T×10, 96T×100
Assay Procedure
1. Prepare all reagents, samples and standards
2. Add 50µ
3. Aspirate and wash 3 times
4. Add 90µ
5. Add 50µ
C
C
L Detection Reagent A to each well. Incubate 1 hours at 37°
L Stop Solution. Read at 450nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L standard or sample and 50µ
2. Add 50µ
3. Aspirate and wash 3 times
4. Add 90µ
5. Add 50µ
C
C
L Detection Reagent A to each well. Incubate 1 hours at 37°
L Stop Solution. Read at 450nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L standard or sample and 50µ
Test Principle
10nm. The concentration of Instant Procollagen I N-Terminal Propeptide (PINP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Procollagen I N-Terminal Propeptide (PINP). Standards or samples are then added to th
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Procollagen I N-Terminal Propeptide (PINP). Standards or samples are then added to th
Precision
CV(%) = SD/meanX100
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Procollagen I N-Terminal Propeptide (PINP) were tested on 3 different plates, 8 replicates in each plate.
Intra-Assay: CV<10%
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Procollagen I N-Terminal Propeptide (PINP) were tested 20 times on one plate, respectively.
Assay procedure summary
1. Prepare all reagents, samples and standards
UniProt ID
P11087