High Sensitive ELISA Kit for A Disintegrin And Metalloprotease 10 (ADAM10)
Ref: CC-HEA766Mu
96T
Información del producto
High Sensitive ELISA Kit for A Disintegrin And Metalloprotease 10 (ADAM10)
Información adicional
Size
48T, 96T, 96T×5, 96T×10, 96T×100
Stabillity at RT
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the
Storage Conditions
-20°C
Shipping Conditions
Entre 4°C y 8°C
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range
18.8-1,200pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 6.7pg/mL.
Specificity
No significant cross-reactivity or interference between High Sensitive A Disintegrin And Metalloprotease 10 (ADAM10) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of High Sensitive A Disintegrin And Metalloprotease 10 (ADAM10).
Research Area
Cardiovascular biology
Enzyme & Kinase
Infection immunity
Tumor immunity
Enzyme & Kinase
Infection immunity
Tumor immunity
Organism species
Mus musculus (Mouse)
Alternative Names
CD156-C
Item Name
A Disintegrin And Metalloprotease 10
Assay Length
3h
Method
Double-antibody Sandwich
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Format
48T, 96T, 96T×5, 96T×10, 96T×100
Assay Procedure
1. Prepare all reagents, samples and standards
2. Add 100µ
3. Aspirate and add 100µ
4. Aspirate and wash 3 times
5. Add 100µ
6. Aspirate and wash 5 times
7. Add 90µ
8. Add 50µ
C
C
C
C
L Stop Solution. Read at 450nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent A. Incubate 1 hour at 37°
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well. Incubate 2 hours at 37°
2. Add 100µ
3. Aspirate and add 100µ
4. Aspirate and wash 3 times
5. Add 100µ
6. Aspirate and wash 5 times
7. Add 90µ
8. Add 50µ
C
C
C
C
L Stop Solution. Read at 450nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent A. Incubate 1 hour at 37°
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well. Incubate 2 hours at 37°
Test Principle
10nm. The concentration of High Sensitive A Disintegrin And Metalloprotease 10 (ADAM10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive A Disintegrin And Metalloprotease 10 (ADAM10). Standards or samples are then a
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive A Disintegrin And Metalloprotease 10 (ADAM10). Standards or samples are then a
Precision
CV(%) = SD/meanX100
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive A Disintegrin And Metalloprotease 10 (ADAM10) were tested on 3 different plates, 8 replicates in each plate.
Intra-Assay: CV<10%
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive A Disintegrin And Metalloprotease 10 (ADAM10) were tested 20 times on one plate, respectively.
Assay procedure summary
1. Prepare all reagents, samples and standards
UniProt ID
O35598