Información del producto
ELISA Kit for Ubiquitin (Ub)
Información adicional
Size
48T, 96T, 96T×5, 96T×10, 96T×100
Stabillity at RT
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the
Storage Conditions
-20°C
Shipping Conditions
Entre 4°C y 8°C
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range
4.94-400ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 1.69ng/mL
Specificity
No significant cross-reactivity or interference between Ubiquitin (Ub) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Ubiquitin (Ub).
Research Area
Developmental science
Neuro science
Reproductive science
Neuro science
Reproductive science
Organism species
Bovine (Cattle), Equus caballus
Canine (Dog), Sus scrofa
Equine (Horse)
Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Canis familiaris
Porcine (Pig), Bos taurus
Canine (Dog), Sus scrofa
Equine (Horse)
Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Canis familiaris
Porcine (Pig), Bos taurus
Alternative Names
Ubiquitin B
Item Name
Ubiquitin
Assay Length
2h
Method
Competitive Inhibition
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Format
48T, 96T, 96T×5, 96T×10, 96T×100
Assay Procedure
1. Prepare all reagents, samples and standards
2. Add 50µ
3. Aspirate and wash 3 times
4. Add 100µ
5. Aspirate and wash 5 times
6. Add 90µ
7. Add 50µ
And then add 50µ
C
C
C
L Stop Solution. Read at 450 nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent A immediately.
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well.
Shake and mix. Incubate 1 hour at 37°
1. Prepare all reagents, samples and standards
2. Add 50µ
3. Aspirate and wash 3 times
4. Add 100µ
5. Aspirate and wash 5 times
6. Add 90µ
7. Add 50µ
And then add 50µ
C
C
C
L Stop Solution. Read at 450 nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent A immediately.
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well.
Shake and mix. Incubate 1 hour at 37°
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ubiquitin (Ub) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ubiquitin (Ub) and unl
Precision
CV(%) = SD/meanX100
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin (Ub) were tested on 3 different plates, 8 replicates in each plate.
Intra-Assay: CV<10%
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin (Ub) were tested 20 times on one plate, respectively.
Assay procedure summary
1. Prepare all reagents, samples and standards
UniProt ID
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