Storage ConditionsStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
SpecificityThis antibody detects endogenous levels of AKT1 only when phosphorylated at Ser473. This antibody also recognizes AKT2 and AKT3 when phosphorylated at the corresponding residues.
Application KeyWB-Ce,IHC-P,ICC,IF
FormLiquid
Recomended DilutionImmunocytochemistry (1:50-1:200) Immunofluorescence (1:50-1:200) Immunohistochemistry (1:50-1:200) Western Blot (1:1000-1:2000) The optimal working dilution should be determined by the end user.
Antigen species Target speciesHuman
ImmunogenOriginal antibody is raised against recombinant AKT1.
Storage BufferIn PBS, pH7.2 (50% glycerol and 0.02% sodium azide)