Información del producto
Rabbit polyclonal antibody raised against full length recombinant GFP. The antibody is conjugated with Fluorescein (FITC).
Información adicional
Size
100 ug
Storage Conditions
Store vial at 4C prior to restoration.
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not
Specificity
wt, rGFP, eGFP. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, anti-Fluorescein and purified and partially purified green fluorescent protein (Aequorea victoria) serum. No reaction was observed against human,
Application Key
WB,IF,ELISA,Flow Cyt
Form
Lyophilized
Recomended Dilution
ELISA (1:20000-1:40000)
Flow Cytometry (1:2000)
Immunofluorescence (1:500-1:2500)
Western Blot (1:10000-1:25000)
The optimal working dilution should be determined by the end user.
Flow Cytometry (1:2000)
Immunofluorescence (1:500-1:2500)
Western Blot (1:10000-1:25000)
The optimal working dilution should be determined by the end user.
Antigen species Target species
Jellyfishes
Immunogen
Recombinant fusion protein corresponding to full length amino acid sequence (246 amino acids) derived from the jellyfish Aequorea victoria GFP.
Storage Buffer
Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% (w/v) sodium azide).
Iso type
IgG
Conjugation
FITC