DYKDDDDK tag polyclonal antibody (HRP)
Ref: AB-PAB31810
DYKDDDDK tag polyclonal antibody (HRP)
Información del producto
Rabbit polyclonal antibody raised against synthetic peptides of FLAG epitope tag. The antibody is conjugated with Horseradish Peroxidase (HRP).
Información adicional
Size
100 ug
Storage Conditions
Store vial at 4C prior to restoration.
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not
Specificity
Carboxy and amino terminal linked FLAG tagged recombinant proteins. This antibody can be used to identify fusion proteins containing the FLAG epitope. The antibody recognizes the epitope tag fused to the amino- and carboxy- termini of targeted proteins. T
Application Key
WB,IHC,ELISA
Immunogen Prot. Seq
DYKDDDDK
Form
Lyophilized
Recomended Dilution
ELISA (1:20000)
Immunohistochemistry (1:500-1:2000)
Western Blot (1:2000-1:5000)
The optimal working dilution should be determined by the end user.
Immunohistochemistry (1:500-1:2000)
Western Blot (1:2000-1:5000)
The optimal working dilution should be determined by the end user.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to DYKDDDDK tag.
Storage Buffer
Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% (w/v) gentamicin sulfate). Do NOT add sodium azide!
Iso type
IgG
Conjugation
HRP