Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human MED1. AA range:641-690
Subcellular Location
Nucleus . A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Human Gen
5469
Observed Band
170kD
Other Name
ARC205
ARC205
Activator-recruited cofactor 205 kDa component
CRSP1
CRSP200
DRIP205
DRIP230
MED1
Mediator complex subunit 1
Mediator of RNA polymerase II transcription subunit 1
PBP
PPARBP
PPARGBP
Peroxiso
RB18A
TRAP220
TRIP2
Background
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. T