Información del producto
Raf-1 (phospho Ser259) rabbit pAb
Información adicional
Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser259. AA range:225-274
Subcellular Location
Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Monkey
Mouse
Rat
Monkey
Mouse
Rat
Human Gen
5895
Observed Band
73kD
Other Name
Proto-oncogene c-RAF
RAF
RAF1
RAF proto-oncogene serine/threonine-protein kinase
Raf-1
cRaf
RAF
RAF1
RAF proto-oncogene serine/threonine-protein kinase
Raf-1
cRaf
Background
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RA