Recomended DilutionFlow Cytometry (0.5-1 ug/million cells in 0.1 mL) Immunofluorescence (0.5-1 ug/mL) Immunoprecipitation (1-2 ug/500 ug protein) (precipitates active CDK1/cyclin B1 complexes) The optimal working dilution should be determined by the end user.